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guinea pig polyclonal anti s100 calcium binding protein β  (Synaptic Systems)
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Overview of expected outcomes (A) Schematic representation of distinct cell populations related to adult hippocampal neurogenesis (AHN). (B–H) Expected staining with anti- (B) vimentin, (C) sex determining region Y-box 2 (Sox2), (D) phospho-histone 3 (PH3), (E) HuC-HuD, (F) Doublecortin (DCX), (G) polysialylated-neural cell adhesion molecule (PSA-NCAM), and (H) calbindin (CB) antibodies. (I) Schematic representation of the components of the human hippocampal neurogenic niche. (J–M) Expected staining with anti- (J) <t>S100</t> <t>calcium-binding</t> protein β <t>(S100β),</t> (K) ionized calcium-binding adaptor molecule 1 (Iba1), (L) phosphorylated γH2A.X (γH2A.X) antibodies, and (M) Ulex Europaeus Agglutinin-I (UEA-1). Yellow scale bar: 20 μm. White scale bar: 10 μm. Yellow triangles indicate positive cells. White triangles highlight the morphology of cells labelled for each marker.
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Overview of expected outcomes (A) Schematic representation of distinct cell populations related to adult hippocampal neurogenesis (AHN). (B–H) Expected staining with anti- (B) vimentin, (C) sex determining region Y-box 2 (Sox2), (D) phospho-histone 3 (PH3), (E) HuC-HuD, (F) Doublecortin (DCX), (G) polysialylated-neural cell adhesion molecule (PSA-NCAM), and (H) calbindin (CB) antibodies. (I) Schematic representation of the components of the human hippocampal neurogenic niche. (J–M) Expected staining with anti- (J) <t>S100</t> <t>calcium-binding</t> protein β <t>(S100β),</t> (K) ionized calcium-binding adaptor molecule 1 (Iba1), (L) phosphorylated γH2A.X (γH2A.X) antibodies, and (M) Ulex Europaeus Agglutinin-I (UEA-1). Yellow scale bar: 20 μm. White scale bar: 10 μm. Yellow triangles indicate positive cells. White triangles highlight the morphology of cells labelled for each marker.
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Overview of expected outcomes (A) Schematic representation of distinct cell populations related to adult hippocampal neurogenesis (AHN). (B–H) Expected staining with anti- (B) vimentin, (C) sex determining region Y-box 2 (Sox2), (D) phospho-histone 3 (PH3), (E) HuC-HuD, (F) Doublecortin (DCX), (G) polysialylated-neural cell adhesion molecule (PSA-NCAM), and (H) calbindin (CB) antibodies. (I) Schematic representation of the components of the human hippocampal neurogenic niche. (J–M) Expected staining with anti- (J) <t>S100</t> <t>calcium-binding</t> protein β <t>(S100β),</t> (K) ionized calcium-binding adaptor molecule 1 (Iba1), (L) phosphorylated γH2A.X (γH2A.X) antibodies, and (M) Ulex Europaeus Agglutinin-I (UEA-1). Yellow scale bar: 20 μm. White scale bar: 10 μm. Yellow triangles indicate positive cells. White triangles highlight the morphology of cells labelled for each marker.
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human s100a9  (Elabscience Biotechnology)
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Overview of expected outcomes (A) Schematic representation of distinct cell populations related to adult hippocampal neurogenesis (AHN). (B–H) Expected staining with anti- (B) vimentin, (C) sex determining region Y-box 2 (Sox2), (D) phospho-histone 3 (PH3), (E) HuC-HuD, (F) Doublecortin (DCX), (G) polysialylated-neural cell adhesion molecule (PSA-NCAM), and (H) calbindin (CB) antibodies. (I) Schematic representation of the components of the human hippocampal neurogenic niche. (J–M) Expected staining with anti- (J) <t>S100</t> <t>calcium-binding</t> protein β <t>(S100β),</t> (K) ionized calcium-binding adaptor molecule 1 (Iba1), (L) phosphorylated γH2A.X (γH2A.X) antibodies, and (M) Ulex Europaeus Agglutinin-I (UEA-1). Yellow scale bar: 20 μm. White scale bar: 10 μm. Yellow triangles indicate positive cells. White triangles highlight the morphology of cells labelled for each marker.
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Overview of expected outcomes (A) Schematic representation of distinct cell populations related to adult hippocampal neurogenesis (AHN). (B–H) Expected staining with anti- (B) vimentin, (C) sex determining region Y-box 2 (Sox2), (D) phospho-histone 3 (PH3), (E) HuC-HuD, (F) Doublecortin (DCX), (G) polysialylated-neural cell adhesion molecule (PSA-NCAM), and (H) calbindin (CB) antibodies. (I) Schematic representation of the components of the human hippocampal neurogenic niche. (J–M) Expected staining with anti- (J) <t>S100</t> <t>calcium-binding</t> protein β <t>(S100β),</t> (K) ionized calcium-binding adaptor molecule 1 (Iba1), (L) phosphorylated γH2A.X (γH2A.X) antibodies, and (M) Ulex Europaeus Agglutinin-I (UEA-1). Yellow scale bar: 20 μm. White scale bar: 10 μm. Yellow triangles indicate positive cells. White triangles highlight the morphology of cells labelled for each marker.
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Overview of expected outcomes (A) Schematic representation of distinct cell populations related to adult hippocampal neurogenesis (AHN). (B–H) Expected staining with anti- (B) vimentin, (C) sex determining region Y-box 2 (Sox2), (D) phospho-histone 3 (PH3), (E) HuC-HuD, (F) Doublecortin (DCX), (G) polysialylated-neural cell adhesion molecule (PSA-NCAM), and (H) calbindin (CB) antibodies. (I) Schematic representation of the components of the human hippocampal neurogenic niche. (J–M) Expected staining with anti- (J) S100 calcium-binding protein β (S100β), (K) ionized calcium-binding adaptor molecule 1 (Iba1), (L) phosphorylated γH2A.X (γH2A.X) antibodies, and (M) Ulex Europaeus Agglutinin-I (UEA-1). Yellow scale bar: 20 μm. White scale bar: 10 μm. Yellow triangles indicate positive cells. White triangles highlight the morphology of cells labelled for each marker.

Journal: STAR Protocols

Article Title: Protocol to study adult neurogenesis in fresh-frozen human hippocampal tissue using an immunofluorescence quantitative approach

doi: 10.1016/j.xpro.2025.104344

Figure Lengend Snippet: Overview of expected outcomes (A) Schematic representation of distinct cell populations related to adult hippocampal neurogenesis (AHN). (B–H) Expected staining with anti- (B) vimentin, (C) sex determining region Y-box 2 (Sox2), (D) phospho-histone 3 (PH3), (E) HuC-HuD, (F) Doublecortin (DCX), (G) polysialylated-neural cell adhesion molecule (PSA-NCAM), and (H) calbindin (CB) antibodies. (I) Schematic representation of the components of the human hippocampal neurogenic niche. (J–M) Expected staining with anti- (J) S100 calcium-binding protein β (S100β), (K) ionized calcium-binding adaptor molecule 1 (Iba1), (L) phosphorylated γH2A.X (γH2A.X) antibodies, and (M) Ulex Europaeus Agglutinin-I (UEA-1). Yellow scale bar: 20 μm. White scale bar: 10 μm. Yellow triangles indicate positive cells. White triangles highlight the morphology of cells labelled for each marker.

Article Snippet: Guinea pig polyclonal anti-S100 calcium-binding protein β (S100β) (1:500) , Synaptic Systems , Cat#287004; RRID: AB_2620025.

Techniques: Staining, Binding Assay, Marker